The Evaluation of Phytochemical and HPLC analysis of Crotalaria angulata , Momordica cymbalaria, Senna hirsuta and Indigofera linnaei and it’s biological applications Phytochemical , HPLC
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To encompass a quantitative evaluation of phytochemical analysis and HPLC analysis of ethanol extracts of Senna hirsuta ; Indigofera linnaei; Crotalaria angulata and Momordica cymbalaria leaves were experimented to have broad analysis on presence of bioactive components. The phytochemical tests showed the bioactive compounds in Senna hirsuta ethanolic extracts with Steroids, Glycosides, Anthraquinones, Saponins Glycosides, Flavonoids and Terpenoids. In Indigofera linnaei, ethanolic extracts of this plant contain Steroids, Glycosides, Saponins, Glycosides and Terpenoids, Anthraquinones, Tannins, Flavonoids and Saponins are absent for this plant. Test for Steroids, Anthraquinones, Tannins and Terpenoids are strongly present in the plant of Crotalaria angulata. The strong presence of Steroids, Glycosides, Tannins, Terpenoids, Saponins foam in the plant of Momordica cymbalaria. The effects of ethanolic extracts of Anti-Bacterial activity of S.hirsuta and Indigofera linnaei with some of bacteria pathogenic strains such as Shigella dysenteriae, Escherichia coli, Salmonella typhi, Proteus vulgaris, Klebsiella pneumoniae and Bacillus subtilis were experimented. The antibacterial activities of the ethanolic extracts were compared favorably with that standard antibiotic (Chloramphenicol). The Ethanolic extract of leaf showed a maximum zone of inhibition (11mm) against Escherichia coli, a Gram negative bacteria. In Indigofera linnaei, the ethanolic extract, show a maximum zone of inhibition (19mm) to Salmonella typhi. In chromatographic technique, the separation and movements of biomolecules has been investigated. Hence, these bio-techniques play a significant role in finding of important material for pharmaceutical industry and have substances that induce a great interest due to their versatile applications. The paper chromatographic technique showed the Rf value at chlorophyll ‘a’ is 0.569 and ‘b’ value 0.123 present in plant Crotalaria angulata. The Rf value at chlorophyll’a’0.569, and ‘b’ value is 0.353 present in the plant Momordica cymbalaria. HPLC analyses allow for the identification of samples of Momordica cymbalaria with peak value of 1676436 and Retention time is 4.092.This particular study revealed the strong quantitative phytochemicals in Crotalaria angulata and Momordica cymbalaria and the same has been found to be the most effective free radical quencher. As a culmination, these plant extracts can be a safe alternative to chemical drugs.
Keywords: Phytochemicals, Biomolecules, Evaluation, Chromatographic. Anti-Bacterial
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